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KMID : 1059520110550020262
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Journal of the Korean Chemical Society
2011 Volume.55 No. 2 p.262 ~ p.267
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Detection of Hepatitis B Virus by LAMP and DHPLC
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Ahn Young-Chang
Seo Jae-Won
Choi Jae-Gu
Jang Won-Choeul
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Abstract
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The denaturing high performance liquid chromatography(DHPLC) with fluorescence detector assay is very useful tool for detecting nucleic acids. Furthermore, loop-mediated isothermal amplification(LAMP) constitutes a potentially valuable tool for rapid diagnosis of pathogenic microorganisms. In this study, we evaluated the specificity, detection limit, and sensitivity of a LAMP method and DHPLC method for rapid detection of the hepatitis b virus(HBV). As a result, the LAMP assay reported here has the advantage of rapid detection whereas, DHPLC assay has more sensitivity than other assays. These findings suggest that LAMP and DHPLC assay may be good tool for rapid diagnosis of clinical HBV infection.
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KEYWORD
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HBV, DHPLC, LAMP, real-time PCR
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